![]() ![]() These cultures, however, are not free from viral contamination. This is one reason for the increasing use of bovine cell cultures. The presence of adventitious viruses in cell cultures is well recognised, and when the cultures are of primate origin there are serious hazards for the production of human viral vaccines. Notes: Usually, the noncytopathic biotype of BVDV was isolated from FBS. Viral contamination of bovine foetal serum and cell cultures. Nuttall,P.A., Luther,P.D., and Stott,E.J. ![]() Usually, the noncytopathic biotype of BVDV was isolated from FBS.ĥ. Our viral screening methods revealed that five of these pretested lots, or 25%, contained endogenous bovine viruses. Of the 51 lots, 20 had been pretested by the suppliers and were considered to be free of known viral contaminants. Over 30% of the lots tested were found to contain bovine viruses they included bovine virus diarrhea virus, parainfluenza type3-like virus, bovine herpesvirus-1, bovine enterovirus type 4, and an unidentified cytopathogenic agent. Fifty-one lots of fetal bovine serum from 14 suppliers were examined. Notes: This investigation employed a viral screening method detect endogenous bovine virus contaminants in commercially supplied fetal bovine serum. Detection of bovine viruses in fetal bovine serum used in cell culture. Kniazeff,A.J., Wopschall,L.J., Hopps,H.E., and Morris,C.S. Notes: "Fetal bovine serum (FBS) frequently contains BVDV or antibodies against BVDV" from Bolin,S.R. A serological study of bovine foetal immunoglobulins. Horner,G.W., Johnson,R.H., Dennett,D.P., and Lane,W.R. Notes: from "Fetal bovine serum (FBS) frequently contains BVDV or antibodies against BVDV(Bolin-SR et.al. A cytopathogenic strain of bovine viral diarrhea-mucosal disease virus isolated from a bovine fetus. Scott,F.W., Kahrs,R.F., and Parsonson,I.M. Notes: "Fetal bovine serum (FBS) frequently contains BVDV or antibodies against BVDV" from Bolin-SR in press 1993.Ģ. Isolation and characterization of viruses from fetal calf serum. Molander,C.W., Kniazeff,A.J., Boone,C.W., Paley,A., and Imagawa,D.T. ![]()
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